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2022 Speakers

Studies on the pollen viability of litchi during storage

Most Marufa Khatun

Most Marufa Khatun, Speaker at Speaker for Plant Biology Congress - Most Marufa Khatun
Bangladesh Agricultural Research Institute, Bangladesh
Title : Studies on the pollen viability of litchi during storage


The litchi (Litchi chinensis) is a delicious, juicy fruit of excellent quality is native to Southern China and Southeastern Asia. Bangladesh is situated within a latitude (20034’ and 26034’ N) and longitude (88001’ and 92041’ E) that is tropical in nature. The availability period of litchi is very short in our country. Improvement programmes are needed for the development of this fruit through hybridization. In general 3 types of flowers, i.e. male, pseudo-hermaphrodite and female, are encountered in litchi; only the female flowers set fruit. The flower initiation differs among the litchi cultivars. So to hybridize, it is necessary to preserve pollen. In case of artificial
pollination, pollen should be collected manually and preserved for future use. The pollen germination ability decrease rapidly after anther dehiscence due to poor storage capability. Storage of viable pollen of litchi for long-term would be highly desirable for breeding programmes. For the improvement litchi crop, the study was conducted during the flowering season 2018 to study the viability of litchi pollen during storage. Male flowers of BARI Litchi-1, BARI Litchi-2, BARI Litchi-3 and BARI Litchi-4 were taken for this study and were collected at
anther dehiscence stage; time between 8 A.M. and 10 A.M. Collected pollens were stored at 5 different storage conditions such as room temperature (25-300C) in petridish and desiccators, refrigerator (5-70C), deep freezer (-20±50C) and deep freezer (-80±50C). In-vitro germination for fresh M1 and M2 pollen showed maximum 37.32% and 66.27% in BARI Litchi-1. In case of room temperature in petridish, pollen viability lasted up to 7 days with very poor viability percent (16.74%) in BARI Litchi-2. Litchi pollen remained viable for 7 days in desiccators. M2 pollen germination was maximum in BARI Litchi-1 (26.89%) and in BARI Litchi-4 (26.39%). In refrigerator (5-70C) storage, litchi pollen can be kept for 60 days. Maximum M2 pollen germination ranged from 24.76% in BARI Litchi-2 to 35.27% in BARI Litchi-1. At deep freezer (-200C±5) storage, litchi pollen stayed viable up to 90 days where the highest (39.65%) M2 pollen germination was obtained from BARI Litchi-1 and minimum (28.85%) germination was observed from BARI Litchi-2. After 12 months storage at deep freezer (-800 C±5), pollen germination rate decreased steadily. It was noticed that, M2 pollen germination was 54.19% in
BARI Litchi-1


Most. Marufa Khatun studied Bachelor Degree in Agriculture from Bangladesh Agricultural University, Mymensingh, Bangladesh; and Master of Science in Horticulture from Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh. She joined as Scientific Officer at Horticulture Research Centre in Bangladesh Agricultural Research Institute (BARI), Gazipur-1701, Bangladesh, in 2006, and promoted to Senior Scientific Officer in 2015, and engaged herself in research for the improvement of important fruit crops emphasizing on their improved production, and propagation technology. The research findings have been published in national journals. She also joined training on litchi improvement from China in 2013.

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