Title : Anatomical Studies on the Effects of Waste Engine Oil Pollutants on the Sataria Barbata and Brachiaria Deflexa Species
The present study explores the use of anatomical characters of S. barbata and B. deflexaas indicators of environmental pollution. 4 kg of air dried soil was measured into 4 kg perforated plastic buckets.Waste engine oil was added to 4kg different soil samples to obtain different concentrations on weight basis: 0% (control), 2%, 4%, 7% and 10% v/w oil-in-soil and allowed to stand for seven days before transplanting. The plants were harvested after 8 weeks of transplanting.The following parameters were measured, anatomical character of the leaves, stems and roots and the epidermal peel of the leaves. The observed changes in the anatomy of the two grass species studied may be due to the waste engine oil polluted soil in which they were grown. The leaves epidermal cells showed that all the two grass species exhibited irregular epidermal cell shape and sinuous cell walls. There was reduction in cell size, the increase in cell shape irregularity, increased thickness of cell wall of the parenchyma tissues and breakdown of vascular bundles as oil concentration increased in stem anatomy. There was reduction in root cell size, the increase in cell shape irregularity and increased thickness of cell wall of the parenchyma tissues and break down of cortex region as oil concentration increased. In conclusion, the two grass species studied were sensitive to waste engine oil contaminated soil which were determined by the five different concentrations of waste engine oil used.