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Patricia Corral-Martínez

Patricia Corral-Martínez_Speaker for Plant Biology Conference 2020
Patricia Corral-Martínez
COMAV (UPV), Spain
Title : From microspore to embryo: how to get stressed and survive to tell

Abstract:

Microspores can be induced to change their developmental pathway and form haploid embryos in vitro. This process, known as microspore embryogenesis (ME), is generally induced by one or more stress treatments. ME can be easily induced in some species, e.g. Brassica napus, while other species are reclacitrant, including the model species arabidopsis and members of the Solanaceae. In B. napus, this change of developmental pathway is commonly induced by a heat stress. After the stress treatment, some microspores start to divide, other adopt a pollen-like development or arrest. Induced cells can either continue to form haploid embryos or arrest and die. We have observed that the fate of these dividing microspores is related to their stress response. Our data indicate that the induced microspores that do not follow further embryo development show endoplasmic reticulum (ER) accumulation (related to ER stress), high lytic activity, accumulation of starch and lipids, loss of cell adhesion. They become callus-like structures, and eventually undergo premature cell death. In embryo-like structures, all these processes are also present but at lower levels, which contributes to survival and conversion into differentiated embryos. Using cellular and molecular approaches we are studying the effect of several stress-related compounds in embryo yield and their role in the occurrence of the above mentioned features in different genotypes with different ME responses. Our aim is to link these cellular processes with stress response and cell fate in different genotypes, in an attempt to understand the underlying mechanisms, design ways to modulate these processes and eventually increase the number of cells committed to embryogenesis.

Biography:

Dr Corral graduated in Biology in 2006, got her MS degree in 2011 (Master in Plant Breeding, COMAV Institute, Polytechnic University of Valencia), and her PhD on July 19th, 2013. The research conducted during her MS degree and PhD was focused on the production of androgenic doubled haploids in different species. After her PhD, Dr Corral was awarded a Marie Slodowska Curie Postdoctoral Individual Fellowship, in the Plant Research International in Wageningen University and Research (WUR). The host group, Embryogenesis lab, supervised for Dr. Kim Boutilier, is focused to understand plant cell totipotency and early cell fate decisions. Currently I am carrying out my second postdoc in the COMAV, developing my own research project as PI.

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