Title : Synthesis of silver nanoparticles using Vaccinium myrtillus L. fruits extract: characterization of particles and study of fungi effect
The production of silver nanoparticles (AgNPs) from bilberry (Vaccinium myrtillus L.) fruits extract was studied. The aqueous 10mM AgNO3 solution when subjected to V. myrtillus extract was bio-reduced and ensued in green synthesis of AgNPs. The aqueous bilberry solution was enriched with polyphenols (6.89mg/g gallic acid equivalent) and flavonoids (1.51 mg/g catechin equivalent). The AgNPs synthesis was manifested by a color change from colorless to yellow brown after the introduction of fruits extract of V. myrtillus. The formation of nanoparticles was monitored spectrophotometrically by measuring the intensity of the surface plasmon resonance band (SPR) of silver. This green procedure has high yields without application of toxic reagents or surfactant template.
As methods of study the AgNPs interactions with environmental microorganisms, like Phanerochaete chrysosporium, the biochemical assays of defense systems of antioxidative activity and proteins contents were applied. The lipid peroxidation, expressed as malondialdehyde content has been also evaluated. Overall, results showed a variation of activity of superoxide dismutase and catalase in fungus mycelium depending on the concentration of AgNPs and the age of fungus after inoculation.