Title: Cryotherapy: Cryogenic Technology for virus elimination from infected plants

Ergun Kaya

Mugla Sitki Kocman University, Turkey


Assoc. Prof. Dr. Ergun KAYA studied Plant Biotechnology at Mugla Sitki Kocman University, Turkey and graduated as PhD in 2011, Gebze Technical University, Turkey. He then went to USDA-ARS, Co, USA for Post-Doc. research. After one-year postdoctoral fellowship supervised by Dr. Dave ELLIS at the National Center for Genetic Resources Preservation, Fort Collins, Co, he obtained the position of an Assist. Prof. Dr. at the Muğla Sitki Kocman University, Turkey. He has published 17 research articles in SCI(E) journals.


Plant viruses are the most harmful pathogens causing the most economically damages and harmful product loses in many plant species. Because of xylem and phloem vessels absence, plant meristem tissues used for meristem cultures are virus-free, but sometimes only meristem cultures are not sufficient for virus elimination. Cryotherapy, a new method based on cryogenic techniques, is used for virus elimination. In this technique, 0.1-0.3mm meristems are excised from organized shoot apex of a selected in vitro donor plant and this meristems are frozen in liquid nitrogen (-196 °C) using suitable criyogenic technique. Cryogenic treatments including physical and chemical dehydration process successfully eliminates plant viruses from infected shoot tips with high frequency. Sanitized shoot tips regenerate plantlets that maintained their virus-free status over time and are micropropagated for successful transfer to the field. In this presentation we aimed to determine cryogenic technologies for virus elimination, compare all cryogenic techniques (vitrification, encapsulation-vitrification, droplet vitrification, two step freezing, dehydration, encapsulation-dehydaration) being the best for different kind of plant species (monocotyledone, dicotylodone, woody, herbaceous etc.) and also indicate critical points (meristem size, explant type, regeneration medium, application of procedures, other culture conditions etc.) effecting regeneration, viability of meristems after cryogenic tratments.