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2024 Speakers

Matskiv Alexandra Olegovna

Matskiv Alexandra Olegovna, Speaker at Plant Science Conferences
Federal Research Center SSC RAS, Russian Federation
Title : Biotechnological method of reproduction of representatives of the genus Actinidia Lindl

Abstract:

Production of high quality planting material is one of the most important directions of modern horticulture development. Representatives of the Actinidia genus in the humid subtropics of Russia are widely spread, but Actinidia deliciosa and Actinidia arguta occupy a special place.  In the Subtropical Research Center, studies on conservation and reproduction of Actinidia varieties and species under in vitro conditions have been initiated. The study of the effect of cytokinins on Actinidia micropropagation showed that the regeneration ability of genotypes and the in vitro multiplication rate are significantly influenced by growth regulators and cultivar characteristics. Depending on the type of growth regulator and its concentration, the varieties Actinidia deliciosa 'Hayward' and 'Monty', and the species A. arguta Planch. showed different morphogenetic potential, i.e. the ability to proliferation of explants and development of microshoots. The optimal composition of growth regulators increasing the multiplication factor was determined: For Actinidia deliciosa 'Hayward' (4.2 pcs/expl.) and 'Monty' (3.8 pcs/expl.) on nutrient medium containing 6 BAP 2 mg/l and GC3 1 mg/l, for A. arguta (4.5 pcs/expl.) on medium with kinetin at a concentration of 1 mg/l.  The influence of growth regulators of auxin nature on the induction of rhizogenesis of 'Hayward' and A. arguta species was studied. Differences in the realization of morphogenetic potential in the studied genotypes under in vitro conditions were revealed. The highest morphometric parameters and percentage of rooting of microshoots (79.8%) for 'Hayward' were observed on medium containing 1 mg/L NUC. For A. arguta species, the best results were obtained when the NUC concentration was reduced twice (93%). It was found that among the studied factors, genetic features have a greater influence on rhizogenesis (51%), the concentration of auxins (AUC, NUC) (23%) and the interaction of factors (21%) have less influence, while the share of influence of random factor amounted to 5%.

The publication was prepared within the framework of the state assignment FGWR-2021-0008, state registration number 122032300347-3.

Biography:

Matskiv Alexandra Olegovna, studied biology at the Peoples' Friendship University of  Russia, was involved in the introduction of small-bulbous plants of the Sochi Black Sea region into in vitro culture, received a specialist diploma in 2012. In 2019, she joined the research group of Samarina Lidia Sergeevna (Federal Research Center of the Subtropical Scientific Center of the Russian Academy of Sciences, Russia, Sochi), studying the patterns and mechanisms of resistance formation in splitting populations of tea (Camellia sinensis (L.) Kuntze) . A search was carried out for genes affecting frost resistance in the collection of Camellia sinensis (L.) Kuntze tea varieties from the Krasnodar region. She has published more than 20 research articles. Currently working on optimizing the rooting process of representatives of the genus Actinidia.

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