Title : Leveraging bacterial isolates for sustainable rice straw decomposition: An eco-friendly paradigm
Annually millions of tons of paddy straw waste produced poses an environmental crisis as the majority of it remains unused or is subjected to polluting burning practices. Microbial composting emerges as a sustainable solution for repurposing rice straw, but it requires robust lignocellulolytic microbes capable of efficient conversion and environmental mitigation. This research focuses on isolation and screening of the potential of microbial isolates to decompose or transform rice straw waste in to pulp across diverse in lab and on field experimental setups. The isolates underwent comprehensive biochemical and molecular characterization. 16s rRNA sequence analysis confirmed the identity of key isolates, including species like Stenothermophil Acinetobacter, Uncultured bacterium, Erwinia chrysanthemi, Vibrio cyclitrophicus, Enterobacter cloacae, Aeromonas sanarellii, Pseudomonas putida, Sphingobacterium thalpophilum strain, and Enterobacter asburiae etc. Results showcased highly efficient rice straw decomposition, with notable achievements such as a 25-day time frame in glass experiments and 35 days in field conditions. Moreover, specific bacterial isolates, such as 5A, TR32, SH2A, SH, S, AK15, AK17, and AK21, demonstrated exceptional efficacy in converting rice straw into pulp. The study also explored laccase activity, a pivotal enzyme in lignin degradation, revealing isolate SH2A as the highest performer. This study promotes eco-friendly alternatives to stubble burning through a multidimensional, sustainable waste management approach, charting a greener and more promising future.
Keywords: Paddy straw waste, lignocellulolytic microbes, sustainable waste management, Laccase.