Title : Protocol optimization for micropropagation of banana varieties(Musa spp.) using shoot-tip culture
Abstract:
Conventional vegetative propagation of bananas generally has low production, the transmission of diseases, and poor preservation of original plant genetic material. Micropropagation is currently the only practical means of achieving rapid, large-scale production of disease-free quality planting material. The present study was conducted to optimize quick and reproducible in vitro micropropagation protocol for three banana varieties (Grandnaine, Poyo, and Butuza) grown in Oromia Experiments on shoot tip culture initiation, shoot multiplication and in vitro rooting were laid out in completely randomized design with 3x2x2, 5x4x3, and 4x3 factorial treatment arrangements respectively. Data were subjected to analysis of variance (ANOVA) and significant means were separated using Duncan's multiple range tests. About shoot multiplication, Grandnaine showed a maximum of 12.67shoots per explant with a 2.17cm shoot length on a medium fortified with 3.5mg/l BAP alone, while Poyo produced a maximum of 13.00shoots per explant with 2.15cm mean shoot length on a medium supplied with 3.0 mg/lBAP and 0.2mg/l IBA. Likewise, Butuza produced a maximumof11.33shootsper explant with a mean shoot length 2.9 cm on medium fortified with 4.0 mg/l BAP + 0.3 mg/l IBA. Half MS -semi-solid medium containing 1.0 mg/l IBA induced the highest rooting with 8 mean root numbers per shoot for Grandnaine. For Poyo, half MS medium supplemented with 1.5mg/l IBA induced the highest rooting response with mean root number per shoot of 7.6. Similarly, ½ MS medium supplemented with 2.0 mg/l IBA induced the highest rooting response with 8 mean root numbers per shoot. In conclusion, this study can be used for rapid and massin Vitro propagation of these three elite Banana varieties.
Keywords: - Micropropagation, Banana Varieties, 6-Benzylaminopurine, acid, Indole butyric acid,
