Plant viruses are the most harmful pathogens causing the most economically damages and harmful product loses in many plant species. Because of xylem and phloem vessels absence, plant meristem tissues used for meristem cultures are virus-free, but sometimes only meristem cultures are not sufficient for virus elimination. Cryotherapy, a new method based on cryogenic techniques, is used for virus elimination. In this technique, 0.1-0.3mm meristems are excised from organized shoot apex of a selected in vitro donor plant and this meristems are frozen in liquid nitrogen (-196 °C) using suitable criyogenic technique. Cryogenic treatments including physical and chemical dehydration process successfully eliminates plant viruses from infected shoot tips with high frequency. Sanitized shoot tips regenerate plantlets that maintained their virus-free status over time and are micropropagated for successful transfer to the field. In this presentation we aimed to determine cryogenic technologies for virus elimination, compare all cryogenic techniques (vitrification, encapsulation-vitrification, droplet vitrification, two step freezing, dehydration, encapsulation-dehydaration) being the best for different kind of plant species (monocotyledone, dicotylodone, woody, herbaceous etc.) and also indicate critical points (meristem size, explant type, regeneration medium, application of procedures, other culture conditions etc.) effecting regeneration, viability of meristems after cryogenic tratments.
Take Away Notes:
• Current presentation will be informative about biotechnological approaches of pathogen elimination from infected plants for the audience.
• The audience will learn different cryogenic techniques and they can compare which application is the best for viability and regeneration after cryotherapy.
• This presentation will provide a practical solution, especially plant biotechnologists and agronomists suffering from plant pathogens can find different and effective solutions in this presentations.