Title : Phenolic profile and in vivo anti-inflammatory activity of aqueous seed extract of date fruit (Phoenix dactylifera L.)
Different diseases provide multiple examples of inflammatory reactions, this process is very variable depending on theiretiology. Usually steroidal and nonsteroidal anti-inflammatory drugs treat the inflammation, however these treatments most often cause undesirable effect. We are interested in natural products. The aim of this study is identified phenolic compounds and to evaluate in vivo anti-inflammatory activity of aqueous seed extract of Phoenix dactylifera L from Algeria. Total phenolic constituents of seed extract was determined by the high-performance liquid chromatography (HPLC-UV Elite LaChrom Hitachi, Japan). Edema induction in mice’s paw by carrageenan at 1%. The aqueouse extract (100, 200, and 300 mg/kg) were administered intragastrically to mice one hour before the subplantar injection of carrageenan. Diclofenac (50 mg/kg) was used as reference standard drug. The major phenolic acids and flavonoids of seed extract identified by HPLC are Protocatechuic, Caffeic and Gallic acids and Catechin, Epicatechin, Daidzein, Chrysin and Rutin. Concerning anti-inflammatory activity, the reduction of paw volume is highly significant (P ≤ 0.001) from the second to sixth hour of the experiment as a response to intragastric administration of aqueous extract at 100mg/kg compared to diclofenac treatment. Our results are confirmed by histological sections of paw skin. It reveals a discreet inflammation with slight leukocyte infiltrate disseminate at some inflammatory site in the group treated with aqueous extract compared to the group treated with Diclofenac. The study suggests a possible correlation between the content of phenolic compounds and the potential antiinflammatory activity of seed date extracts from Algeria.
Key words: Phoenix dactylifera L., phenolic compounds, anti-inflammatory activity, histology in vivo.