Title : Synthesis of silver nanoparticles using Vaccinium myrtillus L. fruits extract: characterization of particles and study of fungi effect
The production of silver nanoparticles (AgNPs) from bilberry (Vaccinium myrtillus L.) fruits extract was studied. The
aqueous 10mM AgNO3 solution when subjected to V. myrtillus extract was bio-reduced and ensued in green synthesis of AgNPs. The aqueous bilberry solution was enriched with polyphenols (6.89mg/g gallic acid equivalent) and flavonoids (1.51 mg/g catechin equivalent). The AgNPs synthesis was manifested by a color change from colorless to yellow brown after the introduction of fruits extract of V. myrtillus. The formation of nanoparticles was monitored spectrophotometrically by measuring the intensity of the surface plasmon resonance band (SPR) of silver. This green procedure has high yields without application of toxic reagents or surfactant template. As methods of study the AgNPs interactions with environmental microorganisms, like Phanerochaete chrysosporium, the biochemical assays of defense systems of antioxidative activity and proteins contents were applied. The lipid peroxidation, expressed as malondialdehyde content has been also evaluated. Overall, results showed a variation of activity of superoxide dismutase and catalase in fungus mycelium depending on the concentration of AgNPs and the age of fungus after inoculation.
Audience Take Away:
• By participating with this paper at the conference in Valencia, the aim is to promote the research carried out at Alexandru Ioan Cuza University in Iasi, the Faculty of Biology/Faculty of Physics in the field of nanotechnologies, more precisely the obtaining and characterization of nanoparticles by biological means. Through this communication there will be the possibility of an in-depth reflection and a mutual exchange of information with researchers from related fields participating in the conference
• The presentation of the results obtained so far at an important international scientific event may attract the attention of potential collaborators for future collaborations with European funding in which our university has a significant role.
• A clear picture will be provided regarding the research activity at our institution, as well as a comparison with the
research process at the participating institutions