Title : CRISPR regulation of plant microbiota : A review
Humans and probiotic bacteria are inseparable for thousands of years. They help us process nutrients better, fight pathogens, and more. Just like us plants have mutualistic relationship with microorganisms. It is well known that plant microbiota is related to plant growth promotion factors and protection against pathogens and other stress factors. Using these interactions for more efficient production to meet the demand will also help to protect the environment. Designing a microbiota that will meet our needs is an important part of the process. Using a molecular approach will be the most efficient way. CRISPR/Cas genome editing tools allows us to make most specific modifications on the microbiome. With these tools we can enhance the capacity of the plant microbiome or alter the plant to benefit more from already existing microbiota.
To use these tools, we first need a good understanding of plant-microbe interactions. We also need a good understanding of modern technologies and bioinformatics. CRISPR/Cas is basically, the immune system of prokaryotes. Components of this immune system can be programmed and can be used for different purposes including genome editing. The main components of CRISPR/Cas are a guide RNA and Cas endonuclease. The important part is protospacer adjacent motif (PAM), a sequence for gRNA to recognise. The rest of the process is easier. The gRNA-Cas endonuclease searches the target sequence along with PAM. After finding their target, they cut the DNA. This allows us to make genetic modifications on any sequence we want with the highest specifity available.
Audience Take Away:
- Audience will be able to design their experiments with CRISPR tools.
- Audience will gain a new insight in most recent methods and technologies.
- Different branches of science can modify this method for their needs.
- This method the most recent method for genome editing.
- This is the most specific method for genome editing and success rate is higher the previous methods.