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2022 Speakers

Bonnin Marie

Bonnin Marie, Speaker at Plant Biotechnology Conferences
University of Corsica, France
Title : Polyploidy and adaptations to environmental stress: physiological, biochemical and genomic determinants of tolerance in citrus polyploid rootstocks


The Mediterranean basin is one of the main citrus production areas in the world. It ranks third behind China and Brazil. In the heart of the Mediterranean, Corsica is located at the northern limit of the citrus production area (40° North). It benefits from pedoclimatic conditions favorable to the cultivation of citrus fruits. However, this citrus cultivation can be endangered by the emergence of strong abiotic constraints. Indeed, the current climate changes result in an increase in the frequency and intensity of stress episodes in plants. In Corsica, these climatic changes can be characterized by episodes of intense drought that force producers to irrigate their crops more intensively. This results in an increase in soil salinity. The increase in salinity leads to a decrease in growth, tree productivity and citrus quality. The selection of rootstocks adapted to these emerging abiotic constraints seems to be a key criterion to facilitate the adaptation of citrus crops. Work carried out by the University of Corsica, CIRAD and INRAE for about ten years now, has shown that tetraploid citrus rootstocks (4x) would be more resistant to nutritional stress, cold and water deficit compared to diploid rootstocks (2x) which are the most commonly used. However, the molecular and genetic basis of this adaptation is not well known, so this thesis aims to study the mechanisms promoting the response to environmental stresses of 2x and 4x citrus rootstocks. It is highly probable that 4x rootstocks show a better tolerance to salt stress but the reasons for this better adaptation remains to be elucidated. To understand and identify the mechanisms involved, we will develop an integrative approach including the study of physiological (photosynthetic capacity, stomatal conductance, chlorophyll fluorescence), biochemical (oxidation markers, specific activity of enzymes involved in the management of oxidative stress), genetic (transcriptome analysis by RNA-seq) and epigenetic (methylome analysis by Bs-seq and Me-Dip-seq) mechanisms. At the end of this work, we will be able to provide new evidence to explain the advantages of tetraploidy over diploidy. We will be able to determine if there is a constitutive preadaptation linked to tetraploidy or if tetraploidy improves the response to stress through phenotypic plasticity.

Audience Takeaway Notes: 

  • Salt stress is an emerging problem of great importance for world agriculture. This research project gives a way of working on how to anticipate the problems related to climate change on crops, because the problem of salinity in arid and semi-arid regions is even more worrying as it is increased by the phenomenon of climate change.

  • The strength of this project is to propose an integrative approach that uses different techniques and different skills (bioinformatics, physiology, biochemistry, molecular biology, genetics). The audience will appreciate the integrative approach of this work.

  • This research project addresses various approaches in several disciplines of biology, such as bioinformatics, physiology, biochemistry and genetics. It is of great interest to academics to demonstrate how to link these disciplines to understand the mechanisms of salt stress response in its entirety.

  • Our integrative approach uses different techniques, and we propose differents experimental setups that could inspire other teams willing to study like us the tolerance of higher plants, in particular perennial plants, to abiotic stress

  • To cope with biotic and abiotic constraints, citrus fruits are grafted on rootstocks selected for their adaptation properties. The increase in salinity in the Mediterranean area associated with climate change requires the development of new rootstocks with better adaptation capacities to salt stress. It is therefore very important to propose an integrative approach that allows the study of physiological, biochemical and genetic determinants of stress tolerance, particularly salt stress in the Mediterranean area.


Currently in Ph.D at the University of Corsica on the project "polyploidy and adaptation to environmental constraints determining physiological, biochemical genetic tolerance of tetraploid rootstocks of citrus". I have a Master degree in Biology and Plant Valorization " of the University of Strasbourg speculation "Molecular Biology and Biotechnology of Plants". In 2018, I joined the team "RNA degradation" at the Institute of Plant Molecular Biology (IBMP). This allowed me to participate in the project "Identification of TUTases involved in viral RNA uridylation of Turnip Mosaic Virus" where I performed a transcriptomic study by RNAseq, using Illumina high-throughput sequencing. In January 2019, my research in Strasbourg focused on the functional study of the UCN endonuclease, a component of Processing bodies (PB) in Arabidopsis thaliana.

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