Title : Harnessing microorganism having potential for biotreatment of wastewater
Abstract:
Determining the diversity of the indigenous microorganism in Palm Oil Mill Effluent (POME) could allow their wider application for the treatment of recalcitrant agro-based wastewater discharge into environment. Many research studies mainly determined the efficiency of microorganism or their co-cultivation with microalgae for enhances treatment of wastewater, suggesting a limited emphasis on the application of microbial diversity. In this study, microorganism was cultured in POME for a period of 15 days using microalgae as source of carbon. Pyrosequencing analysis reveals a diversity of microbial community in 20% (v/v) culture than the control experiment. Most of the bacterial species identified in POME belong to the families of Bacillaceae, Paenibacillaceae, Enterococcaceae, Clostridiaceae, Peptostreptococcaceae, Caulobacteraceae, Enterobacteriaceae, Moraxellaceae, and Pseudomonadaceae. Alpha (α) diversity analysis reveals high composition of microbial community of 52 in both samples. Beta (β) diversity index indicated the occurrence of similar species of microorganisms in unweighted uni fra than theweighted uni fra of both samples. It is therefore suggested that bacteria found from these families could have a potential for synergistic treatment of high strength wastewater generated from palm oil industry.
Key words: Diversity, Microorganism, Wastewater, Pyrosequencing, Palm oil mill effluent
