Title : Polymorphism in SSR-loci associated with E genes in soybean mutant lines perspective for breeding Chebotar Sabina
Abstract:
Detection of alleles of E genes, that are involved in the control of plant response to photoperiod and determination the time to flowering and maturation, with SSR markers could help in the evaluation of adaptive capacity of soybean cultivars in different growth conditions.
With the aim to create new breeding material of soybean (Glicine max (L.) Merrill) the cultivars Femida, Oksana, Podils’ka 416, Zolotysta have been treated by chemical mutagenesis. For this purpose, were used mutagens: D-6, DMSSO-11, DMSSO-12, DMSNPIR-11, DUDMS12, D12DMC-11B, that have been provided by V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry of the National Academy of Sciences of Ukraine (Kiev, Ukraine). After mutagenic treatment lines were growing in experimental field during 5-7 agronomical seasons in Institute of Feeds and Agriculture of Podillia of NAAS (Vinnitsa, Ukraine) and then there were selected perspective for breeding mutant lines.
The aim of our work was to analyse genetic diversity that have been developed in these mutant lines by using microsatellite (MS) markers: Satt_100, Satt_229, Satt_319, Satt_354, Satt_365, Sat_038 that are linked with genes which determined the sensitivity of soybean plants to photoperiod and time to maturation.
In the genotypes of 10 mutant lines, which are perspective for breeding, we have revealed new alleles of microsatellite loci that were not present in parental lines. Totally we have detected 23 alleles of 6 microsatellite loci for this material. Only in 4 cases from 24 (MS-locus/parental genotype) there were not detected changes in allele size for mutant lines in comparing with genotypes of parental cultivars. But this numbers of alleles were less than 35 alleles that we have detected for 19 soybean cultivars also tested also in this project. Among alleleles of mutant lines there were 3 new which are not present among alleles of soybean cultivars.
The results have shown that applied mutagens induce changes in soybean genome and by using these mutagens it is possible effectively increase genetic diversity in loci associated with the genes/loci that determined time of maturity and/or photoperiod sensitivity of soybean. The obtained mutant lines can permit to expand the possibilities of creating soybean varieties with different levels of photoperiodic sensitivity, ripening terms and adaptation ability.
Take Away Notes:
• The mutagens: D-6, DMSSO-11, DMSSO-12, DMSNPIR-11, DUDMS12, D12DMC-11B can be effectively used in chemical mutagenesis of soybean.
• The mutagens: D-6, DMSSO-11, DMSSO-12, DMSNPIR-11, DUDMS12, D12DMC-11B can increase genetic variability in microsatellite loci linked with E – photoperiod sensitivity genes.
• Data from this research could be used by breeders who have the aim to select the most adaptive soybean varieties for geographical latitudes similar to Ukraine conditions since early flowering combined with an extended reproductive stage is preferable for soybean.
• The information about genetic diversity in microsatellite loci that are linked with E genes of Ukrainian cultivars and mutant lines of soybean can be used for comparative genetic investigations.
