Plumbago zeylanica commonly known as ‘Chitrak’ is a medicinal plant which contains a wide range of phytocompounds like flavonoids, alkaloids, napthoquinones, saponins, steroids, tannins, tri-terpenoids, etc. Utilization of in-vitro culture has an advantage over wild type as it possesses high growth rate and independent from seasonal changes. The present investigation was performed to identify presence of different bioactive compounds in three different extracts of in-vitro grown roots of P. zeylanica. Root of the plant was air dried and extracted with methanol, ethanol and chloroform by 72 hours maceration. Seven different phytochemical studies have been conducted i.e. flavonoid, terpenoid, alkaloid, steroid, coumarins, saponin and tannin. Methanolic extract showed presence of flavonoid, terpenoid, steroid and coumarins, ethanolic extract showed presence of terpenoid, steroid, and alkaloid whereas chloroform extract showed presence of only flavonoids and tannins. Further antibacterial activity of different extracts was tested against two bacteria i.e. E.coli and Staphylococcus aureus. Antibacterial activity was determined using zones of inhibition, which were measured by using disc diffusion method. Maximum antibacterial activity was against Staphylococcus aureus. Methanolic extracts of Plumbago zeylanica were found to be more potential for antibacterial activities followed by chloroform and ethanolic extract.
Take Away Notes:
• This study provides the information about application of P. zeylanica in antibacterial activity and shows the presence of different phytocompounds.