Title : Transcriptomic analysis of Moso bamboo seeds during the germination process by RNA-seq
Abstract:
For advanced plant, germination is start point for growth and development, where life activity is most intense. Changing law in germination will contribute to controlling the processing. Moso bamboo is a large, woody bamboo with the highest ecological, economic and cultural value of all the bamboo types, the major genes regulating seed germination in Moso bamboo are not clear.
In this study, Moso bamboo seeds at four stage from their emergence to maturation were selected, the changes of phenotype was observed. There are three important stages in Moso bamboo seed germination:?: cell initiation,?: radicle and germ growth; and III: leaf formation. In addition, we compared the identified genes at different seed developmental stages. Based on the subcategories in biological process, these genes were classified to 14 sub-categories, The cell matrix and membrane, cell differentiation, cell proliferation and gene expression regulation, energy conversion of cells, chloroplasts, ribosome, protein, biochemical component related enzymes, response stimulus etc.
Seed germination is an energy-demanding process, and only sufficient energy can ensure the smooth metabolism. There is less ATP in mature dry seeds. As the seeds absorb water and germinate, various physiological and biochemical activities are active, and the seeds begin to breathe, a large amount of ATP will be produced internally. ATP is produced during respiration and provides the main energy source for the biological reaction during seed germination. Early germination respiration is the core of substance and energy metabolism. There are different respiratory pathways in Moso bamboo seeds, the study found that when the seed respiration pathway changes from EMP/TCA to PPP, it means that the Moso bamboo seeds changes from dormancy to germination. Respiration intensity in the whole germination process of Moso bamboo experiences a gradual upward trend of high → low → high → low → high.
Take Away Notes:
• By the transcriptome analysis to explore the main regulation factors and respiratory pathway on bamboo seed germination.
• This presentation will improve the production efficiency of cultivation and accelerate the breeding process of new varieties of Moso bamboo.
