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2024 Speakers

Nirmalendu Das

Nirmalendu Das, Speaker at Plant Science Conferences
Barasat Government College, India
Title : Application of endobacterial isolates for sustainable production of Oyster mushroom


Oyster mushrooms (Pleurotus spp, ) are very much popular throughout the globe due to their easy cultivation methods and rich nutraceutical contents. They positioned second just after the button mushroom Agaricus bisporus and commercially cultivated in a wide range of temperature and agro-climatic conditions. Though in Agaricus and some other mushroom, association of microbes are reported in various stages of growth and development, however, there are only a few studies on the microbiome of oyster mushrooms. In the present study some endobacteria were isolated from the different parts of sporophore of Pleurotus pulmonarius and their involvement in different stages of growth and  development like vegetative phase, spawning, fruiting body development, and nutraceutical composition was assessed. In vitro production of indole acetic acid, laccase, ammonia, and siderophores are assessed in different endobacterial isolates and the suitable bacterial strains are selected for further study. The selected bacteria were supplemented with spawn, substrate, or both for sporophore production. The bacterial isolates were identified through Gram staining, biochemical characterization, and 16S rRNA sequencing and identified as species of  Priestia and Rossellomorea. The bacterial supplementation with all combinations not only increased the biological efficiency (BE) but also the nutraceutical contents of the mushroom including their antioxidant activities. The bacterial supplementation with substrate/spawn enhanced the BE of the mushroom. The supplementation of endobacterial isolates to enhance oyster mushroom cultivation unwrap a new horizon for sustainable agricultural practices in the mushroom industry. These bacterial isolates  may be utilized in production of probiotics and other secondary metabolites in near future.


Nirmalendu Das has done his research work from IICB, Kolkata and obtained his Ph.D. in 1998 from Jadavpur University, West Bengal, India. He has about 27 years teaching experience and 31 years research experience.  Presently he is working as the Associate Professor of Post graduate department of Botany, Barasat Government College, West Bengal, India. He is the member of several national and international scientific organizations, reviewer of  many international journals and member of research council. He has published more than 45 papers in reputed international  journals in addition to several book chapters of international repute and guiding Ph. D. students.

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