Title : In-vitro antibacterial activity of the Sikkim Himalayan medicinal plant hippophae salicifolia: Promising source of new drug for (Multi Drug Resistance) MDR pseudomonas aeruginosa
Abstract:
Medicinal plants are the most important source of life saving drugs for the majority of the world. Hippophae salicifolia (HS) commonly known as Sea buckthorn, is a versatile plant The present study was carried out to determine the antimicrobial potential of Hippophae salicifolia against Multi Drug Resistant (MDR) Pseudomonas aeruginosa. It is a gram-negative, aerobic, non-spore forming rod that is capable of causing a variety of infections in both immunocompetent and immunocompromised hosts.The Pseudomonas aeruginosa was isolated from clinical specimen and were identified by Gram stain and Biochemical test. The identification of drug resistant gene like (blaCTX-M, blaTEM, blaSHV) by real-time PCR and antimicrobial susceptibility test followed by DNA extraction was done to isolate the MDR Pseudomonas aeruginosa.The extract was further studied on the MDR bacterial strain for their antibacterial properties by agar diffusion followed by MIC and MBC. The result obtained revealed inhibition against P.aeruginosa. The phytochemical test showed the presence of tannins,alkaloid,glycosides, and flavonoid. Therefore it highlights the medicinal plant is a potential source of drug to combat MDR P.aeruginosa at a time when the antibiotics are being rendered ineffective due to emergence of multi drug resistance worldwide.
Keywords: Medicinal plant, Phytochemical test, Multi Drug Resistant (MDR) Pseudomonas aeruginosa, DNA extraction, Drug Resistant gene, Antimicrobial, folkloric medicine.
