HYBRID EVENT: You can participate in person at Singapore or Virtually from your home or work.
2024 Speakers

Pakhawat Pookhamsak

Pakhawat Pookhamsak, Speaker at Plant Biotechnology Conferences
Suranaree University of Technology, Thailand
Title : Pyramiding of powdery mildew and leaf spot resistance genes in mungbean using marker-assisted backcrossing


Mungbean or green gram (Vigna radiata (L.) Wilczek) is one of the important economic crops in Thailand. Protein and several important nutrient sources for human consumption were found in mungbean seeds. However, the current mungbean production is not enough for its requirement due to poor harvest index, lack of genetic variability, susceptibility to abiotic stresses and particularly biotic stresses. Cercospora Leaf Spot (CLS) and powdery mildew (PM) are the major foliar diseases in mungbean production that can reduce the quality and quantity of mungbean yields. CN84-1 with high yield and starch percentage is widely grown in Thailand, but highly susceptible to CLS and PM diseases. In the present study, the pyramiding of 1 CLS and 2 PM resistance genes from donor parents A and B into elite variety CN84-1 was achieved through marker-assisted backcross breeding (MABB). The triple pyramided progenies in each cross from BC1F1 to BC4F1 generations were identified using 6 linked markers to these resistance genes. Using background selection, high percentage of recurrent parent genome (RPG) recovery with a maximum of 97.6 and 100.0% in BC4F1 generation was obtained through 24 and 26 polymorphic ISSR loci of cross A and B, respectively. These results indicate that the pyramided progenies in the advanced generation contain a similar genome with CN84-1 and three resistant genes. Our findings indicate that MABB is an efficient approach to pyramid multiple resistance genes and accelerate backcrossing in mungbean.


Pakhawat Pookhamsak (Ph.D. Candidate) in the School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University Technology, Thailand. Currently, he is a Ph.D. Visiting Research Student at the University of Saskatchewan and Canadian Light Source, Canada. His research activities focused on molecular plant breeding for disease resistance and resistance mechanisms using synchrotron techniques. He has developed several new mungbean breeding lines that contain high yields and are resistant to powdery mildew and leaf spot diseases by using marker-assisted backcross breeding approach.

Signup for updates

By submitting this form, you are consenting to receive emails and notifications from Magnus Group. You can revoke your consent to receive emails at any time by using the Safe Unsubscribe link, found at the bottom of every email