Title : Co2 extract of tobacco (Nicotiána) as antifungal materials of grape diseases
Abstract:
This research investigates the antifungal properties of a carbon dioxide (CO?) extract obtained from tobacco plants cultivated in Kazakhstan. While the primary focus lies on CO? extraction, the study also evaluates its efficacy compared to other extraction techniques in influencing antifungal activity. Given the rising resistance of fungal infections to conventional treatments, there is a growing concern. Furthermore, the study seeks to elucidate the factors impacting the antifungal properties of different tobacco extracts.
Tobacco leaves cultivated in Kazakhstan undergo five distinct extraction processes: heat-assisted extraction, Soxhlet extraction, maceration, supercritical CO?, and subcritical CO? extraction methods. Subsequently, the extracted materials are subjected to testing against various fungal strains, including mildew, powdery mildew, and anthracnose, using standard methods such as micro-dilution and disk diffusion assays. CO? extraction is emphasized as a safe and efficient approach for isolating bioactive compounds from plants.
The study assesses the antifungal activity of the CO? extract against these fungal strains. The minimum inhibitory concentration of each extract is determined to assess its effectiveness in inhibiting fungal growth. Gas chromatography-mass spectrometry (GC-MS) is employed to analyze the chemical composition of the extracts, focusing on key compounds such as Pyridine, 3-(1-methyl-2-pyrrolidinyl); Phytol; 9,12-Octadecadienoic acid, ethyl ester; Vitamin E; Hexadecanoic acid; Cotinine; 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl; Methylamine; N-cyclopentylidene; Pyridine, 3-(3,4-dihydro-2H-pyrrol-5-yl).
The anticipated outcomes of this study include the identification of the most effective method for isolating antifungal compounds from Kazakhstani tobacco. Additionally, the study aims to recognize potential antifungal compounds within the CO? extract and compare their efficacy with alternative extraction methods. The investigation will also explore the impact of terpenes, identified through GC-MS analysis, on the overall antifungal effectiveness of the extract.
Unveiling the most efficient extraction method and understanding the significance of terpenes will provide crucial insights for optimizing the production of innovative, natural antifungal substances derived from Kazakhstani tobacco. This research holds the promise of uncovering alternative strategies for combating fungal infections, particularly those resistant to traditional treatments. Moreover, utilizing locally grown tobacco as a potential source for antifungal compounds could yield notable economic and environmental benefits for Kazakhstan.
