Title : Marker assisted breeding for bacterial blight resistance and yield enhancing gene to improve Pranahitha, a local popular elite fine culture with good cooking quality
Abstract:
Present investigation was conducted to study the marker assisted breeding for Xa21 and Gn1a genes and their phenotypic effect along with yield and yield enhancing characteristics in 50 breeding lines of a three way cross (Pranahitha/MTU1010//Improved Samba Mashuri (ISM)). 50 breeding lines (F4 generation), were screened phenotypically for their resistance against bacterial blight by inoculating with BB culture, among them, twenty nine lines were found to be resistant to bacterial blight possessed with excellent grain yields equivalent to or better than Pranahitha (JGL 11727). In foreground selection, above said twenty nine lines were found homozygous positive for bacterial blight resistance gene, Xa21 (pTA248). 50 breeding lines (F4 generation) were subjected to fore ground selection for yield gene, Gn1a (Gn1a INDEL marker-3), out of these, 36 breeding lines were found to be homozygous positive for Gn1a. These 36 lines were shown excellent grain yields equivalent to or better than female parent, Pranahitha (JGL 11727). In genotypic evaluation (foreground selection) of both traits, BB and grain yield, 21 breeding lines were similarly identified to be homozygous positive for both the genes i.e., Xa21 and Gn1a, eight lines are having only Xa21 resistance allele and 15 lines are having only Gn1a resistant allele. Among these 21 breeding lines, seven lines were having significantly higher yield than the three parents i.e., MTU1010 NIL, Pranahitha (JGL 11727) and Improved Samba Mashuri (ISM) with durable resistance to Bacterial Leaf Blight, which could serve as donors in BB endemic areas and could be released as varieties.
Key Words: Rice, foreground selection, bacterial blight resistance gene (Xa21) and yield enhancing gene (Gn1a)
