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GPB 2025

In vitro propagation of Hyphaene thebaica (l.) mart: A rare and critically endangered high value medicinal plant

Doaa Jamal Sanad Abu Darwish, Speaker at Plant Biotechnology Conferences
National Agriculture Research Center, Jordan
Title : In vitro propagation of Hyphaene thebaica (l.) mart: A rare and critically endangered high value medicinal plant

Abstract:

Hyphaene thebaica (L.) Mart., known as the doum palm, is a highly valuable plant native to Africa, Madagascar, the Middle East, and the Indian subcontinent. This palm species, critically endangered in Jordan, exhibits significant medicinal properties, including anti-inflammatory, antioxidant, antimicrobial, antifungal, and anticancer activities. Despite its extensive use in traditional medicine and nutritional applications, in vitro propagation and callusing techniques for H. thebaica remain unreported. This proposal aims to establish protocols for the micropropagation of H. thebaica, focusing on the optimal explant types, media compositions, and sterilization techniques. The study also seeks to determine the best hormone combinations for callus culture development. By leveraging biotechnological tools, this research intends to multiply and conserve H. thebaica, facilitating the ex-situ production of its valuable secondary metabolites. The outcomes will contribute to preserving this endangered species and harnessing its medicinal potential for pharmaceutical applications.

Audience Take Away Notes:

  • Importance and Medicinal Properties of H. thebaica: The audience will understand the significant medicinal and nutritional benefits of the doum palm, including its anti-inflammatory, antioxidant, antimicrobial, antifungal, and anticancer activities
  • Conservation Challenges and Biotechnological Solutions: The audience will gain insight into the critical conservation status of H. thebaica in Jordan and the potential of biotechnological approaches, specifically in vitro propagation and callusing, to preserve and multiply this endangered species
  • Micropropagation Protocols: The audience will learn about the methodologies and protocols being developed to successfully propagate H. thebaica in vitro, including the selection of explant types, media compositions, sterilization techniques, and hormone combinations for callus culture development
  • Enhanced Conservation Techniques: For those involved in conservation biology or environmental sciences, learning about the in vitro propagation techniques for endangered species like H. thebaica will provide them with advanced methods to preserve and restore threatened plant populations
  • Pharmaceutical and Nutritional Applications: Professionals in the pharmaceutical and nutraceutical industries will gain insights into the medicinal properties and bioactive compounds of H. thebaica, potentially guiding the development of new drugs and health supplements based on its extracts
  • Agricultural and Biotechnological Innovations: Researchers and practitioners in agriculture and biotechnology will benefit from understanding the micropropagation protocols for H. thebaica, which can be applied to other valuable or endangered plant species, enhancing their work in plant tissue culture and genetic conservation
  • Cross-Disciplinary Applications: Faculty from fields such as botany, pharmacology, and biotechnology can build upon this research to explore the medicinal properties and bioactive compounds of H. thebaica further, potentially leading to the development of new therapeutic agents
  • Conservation Strategies: Environmental science and conservation biology faculty can use the methodologies developed in this research to improve conservation strategies for other endangered plant species, enhancing biodiversity preservation efforts
  • Educational Purposes: Faculty involved in teaching plant sciences, biochemistry, or biotechnology can use this research as a case study to illustrate advanced techniques in micropropagation and the application of biotechnological tools in plant conservation and medicinal research
  • Conservation and Restoration Planning: For environmental designers and conservation planners, the research offers practical in vitro propagation techniques that can be used to restore endangered plant species like H. thebaica. This can streamline the process of developing conservation strategies and implementing restoration projects
  • Pharmaceutical and Nutraceutical Product Development: For designers involved in product development within the pharmaceutical or nutraceutical industries, the detailed knowledge of H. thebaica's bioactive compounds and their medicinal properties can guide the design of new health products. This can lead to more efficient formulation and testing processes
  • Sustainable Resource Management: For those working in sustainable agriculture and resource management, the research provides methods to efficiently propagate valuable plant species. This can help in designing sustainable farming practices that ensure a steady supply of medicinal plants without overexploitation of wild populations
  • Accurate Propagation Protocols: By establishing detailed protocols for the in vitro propagation of H. thebaica, the research provides precise guidelines that can be followed to achieve successful cultivation. This accuracy is crucial for designing effective conservation and restoration programs
  • Informative Bioactive Compound Data: The identification of bioactive compounds in H. thebaica and their medicinal properties offers new information that can be used in the design of pharmaceutical and nutraceutical products. This helps in accurately formulating products with desired therapeutic effects
  • Optimized Growth Conditions: Understanding the optimal growth conditions, including explant types, media compositions, and sterilization techniques, provides essential information for designing efficient and reproducible tissue culture systems. This can assist researchers and practitioners in refining their methodologies for better outcomes
  • Biodiversity Conservation: By providing techniques for the propagation and conservation of H. thebaica, this research supports efforts to maintain biodiversity, especially for critically endangered species
  • Economic Benefits: The successful propagation and cultivation of H. thebaica can lead to economic benefits for local communities through the sustainable harvesting and sale of its fruits and other plant parts, which have medicinal and nutritional value
  • Cultural Preservation: The research contributes to preserving the cultural heritage associated with H. thebaica, which has historical significance in ancient Egyptian and African civilizations
  • Scientific Knowledge Expansion: The study adds to the scientific literature on plant tissue culture, micropropagation, and the medicinal properties of lesser-known plant species, providing a foundation for further research and exploration
  • Sustainable Development: By promoting the use of biotechnological tools for plant conservation and medicinal research, the study supports sustainable development goals, particularly those related to health, environmental sustainability, and biodiversity conservation
  • Educational Resource: The research can serve as an educational resource for students and educators in fields such as botany, pharmacology, biotechnology, and environmental science, providing a practical example of applied research and conservation techniques
  • Enhanced Research Collaboration: The findings and methodologies from this research can foster collaborations between academic institutions, research organizations, and industry partners, leading to multidisciplinary projects and innovations
  • Improved Agricultural Practices: Insights gained from the micropropagation of H. thebaica can be applied to other valuable or endangered plant species, leading to improved agricultural practices and crop management strategies
  • Health Benefits: The exploration of H. thebaica's medicinal properties could lead to the development of new treatments for various health conditions, contributing to public health improvements
  • Climate Change Mitigation: By developing strategies to conserve and propagate plants that are threatened by climate change, the research helps mitigate the adverse effects of climate change on biodiversity

Biography:

Dr. Doa’a Abudarwish pursued her Biology studies at the University of Jordan, Amman, graduating with a Master's degree in 2014. Subsequently, she became a part of the research group in the plant tissue culture department at the Hamdi Mango Center for Scientific Research. In 2023, she earned her Ph.D. from the same institution. Post-graduation, she took on a role as a researcher in the plant tissue culture department at the Plant Biotechnology Directorate within the National Agriculture Research Center (NARC).

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